SCL 4.1/F7

93031204, rat nerve (sheath), Flattened or crescent shaped

• UNSPSC代码: 41106514

属性

• 产品名称: SCL 4.1/F7, 93031204
• 生物来源: rat nerve (sheath)
• 生长模式: Adherent
• 核型: 42,XY / 43,XY+7
• 形态学: Flattened or crescent shaped
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Rat Schwann cell

细胞系描述

This cell line was derived from neonatal Wistar rat Schwann cells being cultured for several months with intermittent exposure to the mitogen, cholera toxin and infrequent passaging to avoid premature transformation. After cloning one of these cultures by limiting dilution, the line SCL4.1/F7 was established growing without mitrogen. The cells display a subratum adhesion which is calcium-dependent in the millimolar range and pronounced contact-inhibition of growth. Confluent or subconfluent cultures readily cease proliferation and change to a differentiated (stellate / bipolar) morphology secreting an autocrine antiproliferative factor. Cells have been eradicated from mycoplasma at ECACC. With increasing passage cells have been shown to undergo transformation. It is recommended to growth-arrest cells for a few days before subculture to avoid rapid transformation.

培养基

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split semi-confluent cultures 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C Saturation density is 10,000 cells/cm².

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.