U28

Reactivity: Anti-IgG2a, mouse, 93022518

• 分類程式碼代碼: 41106514

属性

• 生物源: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• 骨髓瘤: Sp2/0
• 反應: Anti-IgG2a, mouse
• 技術: cell culture | mammalian: suitable
• 同型: IgM
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse Sp2/0 x Mouse BALB/c spleen

細胞系描述

The hybridoma U28 was obtained by fusing SP2/0 myeloma cells with spleen cells from BALB/c mice that had been given repeated intranasal infection with a single i.v. injection before fusion. The antibody binds murine IgG2a of "a" allotype and the Fc region. There is no cross reactivity with A/PR/8/34 influenza virus, DNP, NP or DNA cytoskeletal proteins. Contains a CH2 domain.

免疫原

A/PR/8/34 mouse pathogenic human influenza virus

應用

Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantification

培養基

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

其他說明

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