46Br.1G1

92091814, human skin, Fibroblast

• UNSPSC代码: 41106514

属性

• 产品名称: 46Br.1G1, 92091814
• 生物来源: human skin
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Fibroblast
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human skin fibroblast, SV40 T antigen transformed

细胞系描述

Derived from 46BR, a fibroblast from an individual with hypogammaglobulinaemia. The parent line was transformed with the plasmid pSV3gpt expressing SV40 T-antigen.

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 15% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; EDTA; 5% CO₂; 37°C.

其他说明

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