HT29 gluc C1

92012401, human colon, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: HT29 gluc C1, 92012401
• 生物源: human colon
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Elevated levels of cystic fibrosis transmembrane conductance regulator (CFTR) protein
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian colon adenocarcinoma

細胞系描述

A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.

培養基

DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO₃) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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