PI.1 Ut

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: raccoon uterus
• 包装: tube of 5 μg 90111909-DNA-5UG; pkg of vial of cells 90111909-1VL
• 生长模式: Adherent
• 核型: 2n = 38
• 形态学: Fibroblast-like
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Racoon uterus

细胞系描述

Derived from trypsinised uterine tissue of an adult female Racoon. Pl.1Ut was developed for studies of feline and canine viruses since the Racoon is one of the few animals which is susceptible to both.

应用

Virus studies: feline and canine viruses

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm² by washing 3 times with 0.255 trypsin or trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan; maximum 90 passages expected. Growth rate is very slow.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.