SW 1417

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90102543, human colon, Epithelial

• UNSPSC代码: 41106514

属性

• 生物来源: human colon
• 包装: tube of 5 μg 90102543-DNA-5UG; pkg of vial of cells 90102543-1VL
• 生长模式: Adherent
• 核型: 2n = 46
• 形态学: Epithelial
• 产品: Carcinoembryonic antigen (CEA)
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human colon adenocarcinoma

细胞系描述

Derived from a grade III adenocarcinoma of the colon in a 53 year old female. CEA is produced and electron microscopy reveals the presence of brush borders around colonies in vitro.

应用

Tumourigenicity studies

培养基

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; No CO₂; 37°C. Centrifugation and resuspension are recommended to disperse clumps.

其他说明

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