L-M (TK-)

90083001, mouse connective tissue, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: L-M (TK-), 90083001
• 生物源: mouse connective tissue
• 增長模式: Adherent
• 染色體組型: Modal no. 45; hyperdiploid
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse C34/An connective tissue

細胞系描述

Derived from a sub-line of a BUdR resistant strain of the L-M mouse fibroblast cell line. This cell line has potential for use in genetic and biochemical studies including somatic cell hybridisation and gene transfer.

應用

Gene transfer, genetic, biochemical and hybridisation studies

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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