K562 Cell line human

89121407, human blood (chronic myelogenous leukemia), Lymphoblast

• 别名: K-562 Cells
• 分類程式碼代碼: 41106514

属性

• product name: K562 Cell line human, 89121407, from human blood(chronic myelogenous leukemia)
• 生物源: human blood (chronic myelogenous leukemia)
• 增長模式: Suspension
• 染色體組型: 2n = 46
• 形態學: Lymphoblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian chronic myelogenous leukaemia

細胞系描述

Established from pleural effusion of 53 year old female with chronic myelogenous leukaemia in terminal blast crisis. Population highly undifferentiated and of the granulocytic series. Can be used as a highly sensitive target for the in vitro natural killer assay. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series.

應用

K562 cell line has been used to study the protecting role of cluster of differentiation-39 (CD39) attenuation against DNA damage. It has also been used to study the relevance of a novel sorting approach for separating cells, called “Slice and Dice Sorting”.

Natural killer assay and tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 8
D16S539: 11,12
D5S818: 11,12
D7S820: 9,11
THO1: 9.3
TPOX: 8,9
vWA: 16

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO₂; 37°C.

其他說明

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