A6

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: toad kidney
• 包装: tube of 5 μg 89072613-DNA-5UG; pkg of vial of cells 89072613-1VL
• 生长模式: Adherent
• 核型: Hyperdiploid
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

South African clawed toad kidney

细胞系描述

Derived from normal kidneys of an adult male toad (Xenopus laevis). A6 cells support the replication of Granoff′s frog viruses but not the Lucke inclusion-tumour associated virus.

应用

Replication studies

培养基

NCTC 109 + 15% Distilled H₂O + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 27-28°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.