P22

89030317, hamster ovary, Epithelial

• UNSPSC代码: 41106514

属性

• 产品名称: P22, 89030317, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• 生物来源: hamster ovary
• 包装: tube of 5 μg 89030317-DNA-5UG; pkg of vial of cells 89030317-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Hamster Chinese ovary

细胞系描述

Chinese hamster ovary cell line which lacks ornithine decarboxylase activity.

培养基

EMEM (EBSS) + Ham′s F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.1% BSA.

传代培养常规

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.1% cold PBS and 0.25% cold trypsin. Place flasks at 4°C for 5-10 min. or until cells detach; 5% CO₂; 37°C. Pre-treat culture flasks with porcine gela

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.