CNC 127I

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: mouse breast
• 包装: tube of 5 μg 87091701-DNA-5UG; pkg of vial of cells 87091701-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Fibroblast
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse RIII mammary tumour

细胞系描述

Subclone of parent line C127. The cells support the growth of Bovine Papilloma Virus and have been used for DNA transfection studies.

应用

Transfection and virus studies: Bovine papilloma virus

培养基

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.