SW 403

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87071008, human colon, Epithelial

• UNSPSC代码: 41106514

属性

• 生物来源: human colon
• 包装: tube of 5 μg 87071008-DNA-5UG; pkg of vial of cells 87071008-1VL
• 生长模式: Adherent
• 核型: 2n = 46, triploid
• 形态学: Epithelial
• 产品: Carcinoembryonic antigen (CEA)
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human Caucasian colon adenocarcinoma

细胞系描述

Established from a grade III adenocarcinoma of the bowel of a 51 year old Caucasian female. The cells produce CEA and are tumorigenic in nude mice. Electron microscopy reveals prominent golgi apparatus.

应用

Tumourigenicity studies

培养基

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin/EDTA; No CO₂; 37°C. Cells do not reach confluency but will grow in small islands.

其他说明

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