Ehrlich-Lettre Ascites strain E cell line

87032503, mouse unknown/unspecified, Epithelial

• UNSPSC代码: 41106514

属性

• 产品名称: Ehrlich-Lettre Ascites strain E cell line, from mouse unknown/unspecified, 87032503
• 生物来源: mouse unknown/unspecified
• 生长模式: Adherent
• 核型: 2n = 40
• 形态学: Epithelial
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse carcinoma

细胞系描述

Established from the explant of a 7 day old tumour from the parental Ehrlich-Lettre Ascites carcinoma. Prior to development, strain E was subjected to a schedule of seven mouse passages and six in vitro passages over a period of 17 months. The cells exhibit an unusually high mean chromosome number and possess the marker chromosomes present in the parental Ehrlich tumour. This cell line may be of use in comparative testing of anti-tumour agents in vivo and in vitro.

应用

Anti-tumour test and tumourigenicity studies

培养基

NCTC 135 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change 3 times a week.

其他说明

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