AC2

86061902, mouse blood, Lymphoblast

• UNSPSC代码: 41106514

属性

• 产品名称: AC2, 86061902
• 生物来源: mouse blood
• 包装: tube of 5 μg 86061902-DNA-5UG; pkg of vial of cells 86061902-1VL
• 生长模式: Suspension
• 核型: Not specified
• 形态学: Lymphoblast
• 产品: Not specified
• 受体: Interleukin 3 (IL-3)
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse lymphoblast

细胞系描述

Derived from a mouse basophil clone and is dependent on Interleukin-3 (IL-3) for growth. IL-3 may be added in the form of Wehi conditioned media (10-20%). Culture densities between 10,000 and 100,000 cells/ml is necessary to retain cells in exponential growth phase.

应用

Growth factor studies, IL-2 assays

培养基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 10,000 to 100,000 cells/ml; 5% CO₂; 37°C. Cells will rapidly die at high densities above 5x100,000 cells/ml. Culture very sensitive to agitation.

其他说明

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