CCRF-HSB-2

85112801, human blood, Lymphoblast

• 分類程式碼代碼: 41106514

属性

• product name: CCRF-HSB-2, 85112801
• 生物源: human blood
• 增長模式: Suspension
• 染色體組型: 2n = 46
• 形態學: Lymphoblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian acute lymphoblastic leukaemia

細胞系描述

A T lymphoblastoid line obtained from the peripheral blood of an 11 year old Caucasian male with acute lymphoblastoid leukaemia which has been passaged eight times through new-born Syrian Hamsters. These may be grown to a high density in a spinner type suspension culture.

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 9,13
D5S818: 11,12
D7S820: 11,12
THO1: 9,10
TPOX: 8
vWA: 18,19

培養基

IDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 5-8x100,000 cells/ml; 5% CO₂; 37°C. Maximum cell density 2-3x1,000,000 cells/ml.

其他說明

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