生物来源
Rat x Rat Hybridoma
包装
tube of 5 μg 85102271-DNA-5UG
pkg of vial of cells 85102271-1VL
ImmunoDon
AO rat spleen
骨髓瘤
YB2/3.0 Ag20
反应性
Anti-Glycoprotein of Chlamydomonas reinhardii
技术
cell culture | mammalian: suitable
同位素/亚型
IgM
运输
dry ice
储存温度
−196°C
细胞系来源
Rat YB2/3.0 Ag20 x Rat AO spleen
细胞系描述
The antibody recognises the glycoprotein 2BII of Chlamydomonas reinhardii. Binding is lost after acid hydrolysis or de-glycosylation of 2BII. AFRC MAC 9 shows similar specificity and properties to AFRC MAC 6, 7, 8, 10 and 11.
免疫原
2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii
培养基
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
传代培养常规
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation
其他说明
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