BHK TK-

85011423, hamster kidney, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: BHK TK-, 85011423
• 生物源: hamster kidney
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Hamster Syrian kidney

細胞系描述

BHK TK- is a derivative of BHK21 (clone 13) rendered TK- by selection in 30μg/ml BUdR. Used widely for research on viruses and on neoplastic transformation especially with polyoma virus.

應用

Transformation and virus studies

培養基

DMEM or GMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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