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生物来源
Mouse x Rat Hybridoma
包装
tube of 5 μg 13082218-DNA-5UG
pkg of vial of cells 13082218-1VL
ImmunoDon
Rat PVG spleen
骨髓瘤
NS1
反应性
Mouse CD22
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
细胞系描述
The hybridoma cell line OX-97 secretes monoclonal IgG1 antibodies reactive with mouse CD22 domain 2.
免疫原
Recombinant CD22
应用
The isotype of the monoclonal antibody produced by this cell line is rat IgG1.
培养基
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)
传代培养常规
Seed at 5 x 104 cells/ml (5 x 105 cells/ml at resuscitation). Subculture when cells reach 5 x 105 - 1 x 106 cells/ml (usually after 3-4 days), 5% CO2, 37°C. 20% FBS can be used at resuscitation.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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