2fTGH cell line human

12021508, human fibrosarcoma, Epithelial

• UNSPSC代码: 41106514

属性

• 产品名称: 2fTGH cell line human, 12021508
• 生物来源: human fibrosarcoma
• 生长模式: adherent
• 形态学: Epithelial
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系描述

The human sarcoma cell line HT 1080 (ECACC Catalogue number 85111505) was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by alpha interferon to create the 2fTGH cell line, enabling the selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the parent 2fTGH cell line enabled the isolation of a number of IFNg signalling mutants available from ECACC: U2A (12021502), U3A (12021503), U4A (12021504), U4C (12021505), U5A (12021506), U6A (12021507). Each contains a different mutation in the IFN signalling pathway. The 2fTGH panel provides a tool for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway.

DNA图谱分析

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19

培养基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4 x 10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.