JU77 cell line human

10092309, human lung, epithelioid: spindly with few vacuoles. Cobblestone morphology at confluency.

• 分類程式碼代碼: 41106514

属性

• product name: JU77 cell line human, 10092309
• 生物源: human lung
• 描述: Malignant mesothelioma cells from human lung
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial-like: cells are spindle-shaped with few vacuoles. Upon confluence develop an epithelioid "cobblestone-like mat"
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human malignant mesothelioma

細胞系描述

This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. JU77 is not tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0140

應用

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

培養基

RPMI 1640 (with 2mM L-Glutamine+ 25mM HEPES) + 5% FCS

例行更新培養

Optimal split ratio 1:8 (seeding density 0.8 x104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

其他說明

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Richard Lake - University of Western Australia, Australia