N1E-115-1

8062511, mouse nerve, neuronal

• 分類程式碼代碼: 41106514

属性

• product name: N1E-115-1, 08062511
• 生物源: mouse nerve
• 增長模式: Adherent
• 染色體組型: modal no. of chromosomes is 192 see reference
• 形態學: neuronal
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse neuroblastoma

細胞系描述

A subclone of the cell line N1E-115 established by Amano et al. The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300. (Mouse strain A/Jax). N1E-115 cells are reported to be slightly contaminated with with a cell type of the morphology of C6 glioma cells. It is recommended to use N1E-115-1, a subclone of N1E-115, that is devoid of the contamination.

應用

can be used as a neuronal model

培養基

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Population doubling approximately 20 hours.

其他說明

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