NS20Y-BU-4

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: mouse nerve
• 包装: tube of 5 μg 08062503-DNA-5UG; pkg of vial of cells 08062503-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Neuronal
• 产品: Choline acetyltransferase
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse neuroblastoma

细胞系描述

A bromodeoxyuridine-resistant mutant of NS20Y (Sigma Catalogue number 08062517) The cell line NS20Y is a cholinergic cell line derived from mouse neuroblastoma C1300 Mouse strain A/Jax.

应用

Used in the generation of cell hybrids.

培养基

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)+ 1 mM 8-bromodeoxyuridine. Cells can also be grown in the absence of bromodeoxyuridine

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.