HeLa (AC-free)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: human cervix
• 包装: tube of 5 μg 08011102-DNA-5UG; pkg of vial of cells 08011102-1VL
• 生长模式: Aggregates in suspension
• 核型: 2n = 46, aneuploid
• 形态学: Spherical
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human cervical carcinoma, serum-free, animal component (AC) free

细胞系描述

HeLa cell line (Sigma catalogue no. 93021013) adapted to grow in animal component free medium (EX-CELL HeLa Serum-Free Medium for HeLa Cells Sigma cat no 14591C).

应用

Virus studies

培养基

EX-CELL HeLa Serum-Free Medium (Sigma cat no 14591C)+ 2mM Glutamine. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

传代培养常规

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.