22Rv1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 5092802, human prostate, Epithelial

• 别名: 22Rv-1 Cells, CWR-22rv1 Cells, CWR22-R1 Cells
• UNSPSC代码: 41106514

属性

• 生物来源: human prostate
• 包装: tube of 5 μg 05092802-DNA-5UG; pkg of vial of cells 05092802-1VL
• 生长模式: Adherent aggregates
• 核型: 49,XY,del(1)(p10),+i(1)(q10),der(2)t(2;4)(p13;q31)del(2)(q13q33),der(4)t(2;4)(p13;q31),t(6;14)(q15;q32),+7,+12[5]/50,idem,+3[1]
• 形态学: Epithelial
• 产品: Not specified
• 受体: androgen receptor positive
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human Xenograft Prostate

细胞系描述

22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. The cell line expresses prostate specific antigen (PSA). Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis. Growth is stimulated by epidermal growth factor (EGF) but is not inhibited by transforming growth factor β-1 (TGF β-1). This cell line is tumorigenic in nude mice.

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 6,9.3
TPOX: 8
vWA: 15,21

培养基

RPMI 1640 without phenol red (Sigma R7509) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

传代培养常规

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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