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Merck
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主要文件

Vero-Hektor

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

UNSPSC代码:
41106514

生物来源

African green monkey kidney

包装

tube of 5 μg 03092503-DNA-5UG
pkg of vial of cells 03092503-1VL

生长模式

Adherent

核型

Not Specified

形态学

Fibroblast-like

受体

Not specified

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相关类别

细胞系来源

African green monkey kidney cells, serum-free

细胞系描述

Vero cell line (Sigma catalog no. 84113001) adapted to grow in the chemically defined protein- and peptide-free medium Hektor G (Cell Culture Technologies). This cell line is substrate dependent, i.e., adherent and may be suitable for the replication of viral particles.

培养基

Hektor G medium (Cell Culture Technologies http://www.cellculture.com/) + 4 mM L-Glutamine (G7513). When freezing cells down use Hektor G medium + 4 mM L-Glutamine (G7513) + 10% DMSO (D2650).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm2. Use EDTA only (no trypsin); 5% CO2; 37 °C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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