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用途
sufficient for 200 tests
质量水平
包装
pkg of 1 kit
技术
flow cytometry: suitable
应用
cell analysis
detection
检测方法
fluorometric
运输
wet ice
储存温度
2-8°C
相关类别
特点和优势
相比于TUNEL等基于DNA的检测方法，该方法能更早发现细胞凋亡进程。
- 快速标记细胞。只需10分钟即可完成细胞染色。
- 无需细胞固定或预处理，缩短检测时间并允许在后续研究中继续使用细胞。
- 附带6-羧基荧光素二醋酸盐二级染料，可从活细胞和坏死细胞中区分凋亡细胞。
原理
Sigma的Annexin V-Cy3试剂盒可以配合荧光显微镜来检测凋亡细胞上结合的膜联蛋白V。该试剂盒采用Cy3.18作为偶联膜联蛋白的荧光染料。通过显微镜检测发现，Cy3.18的荧光亮度比FITC偶联物更高。该试剂盒包含非荧光化合物6-羧基荧光素二醋酸盐 (6-CFDA)，后者可以进入到细胞并被活细胞中的酯酶水解，形成荧光化合物6-羧基荧光素，从而证明细胞是否存活。结合荧光显微镜，就可以区分出早期凋亡细胞（膜联蛋白V阳性，6-CFDA阳性）、坏死细胞（膜联蛋白V阳性，6-CFDA阴性）和活细胞（膜联蛋白V阴性，6-CFDA阳性）。
法律信息
Cy3 is a trademark of Cytiva
仅试剂盒组分
产品编号
说明
- Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Christo Paul Preethy et al.
Journal of pharmacology & pharmacotherapeutics, 3(1), 26-34 (2012-03-01)
To find the efficacy of serial extracts of Anisomeles malabarica in inhibiting proliferation of and inducing apoptosis in human cervical cancer cells, SiHa and ME 180, that are HPV 16-positive. The whole plant was extracted in n-hexane, chloroform, ethyl acetate
Kasturi Muthoosamy et al.
Scientific reports, 6, 32808-32808 (2016-09-07)
Metastasis of lung carcinoma to breast and vice versa accounts for one of the vast majority of cancer deaths. Synergistic treatments are proven to be the effective method to inhibit malignant cell proliferation. It is highly advantageous to use the
Rajeev Kumar Pandey et al.
PloS one, 6(2), e17093-e17093 (2011-02-25)
Mycobacterium indicus pranii (MIP), also known as Mw, is a saprophytic, non-pathogenic strain of Mycobacterium and is commercially available as a heat-killed vaccine for leprosy and recently tuberculosis (TB) as part of MDT. In this study we provide evidence that
C Pigault et al.
Journal of molecular biology, 236(1), 199-208 (1994-02-11)
Annexins are intracellular proteins which bind to membranes in a Ca(2+)-dependent manner and which have been proposed to play regulatory roles in different membrane processes. In the present study, the stoichiometry of the Ca(2+)-dependent binding of annexin V to phosphatidylserine
Mohamed Asik R et al.
Materials science & engineering. C, Materials for biological applications, 103, 109840-109840 (2019-07-28)
Development of novel approach for cancer therapy, sparing healthy normal cells overcoming the limitation of available therapies is of prime importance for cervical cancer treatment. Recently metal oxide based chemotherapeutics has emanated as a promising approach for cancer therapy. Hence
商品
细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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