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Merck

ADD620C

Sigma-Aldrich

HepaRG Maintenance and Metabolism Medium Supplement with Antibiotics

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

形狀

liquid

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

Biopredic′s HepaRG Maintenance and Metabolism Medium Supplement with Antibiotics can be used with all HepaRG cell lines. See user guide for detailed protocols.
HepaRG cells uniquely maintain significant levels of hepatic cell functions, being CYP450 inducible and support the complete hepatitis B virus (HBV) replicative cycle.

應用

HepaRG maintenance and metabolism medium supplement with antibiotics has been used to culture HepaRG cells:
  • to study the interaction of hepatocytes and cholangiocytes when challenged with chlorpromazine (CPZ)
  • to assess the validity of stable reference genes (RG) for gene expression using quantitative real-time polymerase chain reaction (RT-qPCR)
  • as a supplement in basal hepatic cell culture medium to study the effects of cholestasis on liver connexins

法律資訊

Use of this product is subject to one or more license agreements.

HepaRG cells are patented and their use is strictly limited; consider the cells as a single use, disposable product that must be destroyed upon conclusion of a study or experiment. Propagating, reproducing, cloning, subcloning or any other use of the cells following the conclusion of a study is prohibited. Use of the cells to produce or manufacture commercial products for general sale or for use in the manufacture of products intended for general sale is prohibited. Transfer of the cells to anyone not employed within the same organization, whether for financial benefit or not, is prohibited. If you are unwilling to accept the terms of this LIMITED USE LICENSE, do not ORDER or use them and immediately return the cells for credit. Violators of this Limited Use Licesense will be prosecuted to the fullest extent of the law.
HepaRG is a trademark of BioPredic International company

象形圖

Health hazard

訊號詞

Warning

危險聲明

危險分類

Repr. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Axelle Cooreman et al.
International journal of molecular sciences, 21(18) (2020-09-11)
Connexins are goal keepers of tissue homeostasis, including in the liver. As a result, they are frequently involved in disease. The current study was set up to investigate the effects of cholestatic disease on the production of connexin26, connexin32 and
Katie Morgan et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 111, 1408-1416 (2019-03-08)
Chlorpromazine (CPZ) is a neuroleptic drug and prototype compound used to study intrahepatic cholestasis. The exact mechanisms of CPZ induced cholestasis remain unclear. Rat hepatocytes, or a sandwich culture of rat and human hepatocytes, have been the most commonly used
Joanna Brzeszczyńska et al.
Cells, 9(3) (2020-04-05)
Gene expression analysis by quantitative real-time polymerase chain reaction (RT-qPCR) is routinely used in biomedical studies. The reproducibility and reliability of the data fundamentally depends on experimental design and data interpretation. Despite the wide application of this assay, there is
Catherine C Bell et al.
Drug metabolism and disposition: the biological fate of chemicals, 45(4), 419-429 (2017-02-01)
Reliable and versatile hepatic in vitro systems for the prediction of drug pharmacokinetics and toxicity are essential constituents of preclinical safety assessment pipelines for new medicines. Here, we compared three emerging cell systems-hepatocytes derived from induced pluripotent stem cells, HepaRG
Maxime Demazeau et al.
International journal of pharmaceutics, 524(1-2), 268-278 (2017-04-04)
In this study, we evaluated cationic liposomes prepared from diether-NH

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