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Merck

A7674

Sigma-Aldrich

Anti-AOP1 antibody, Mouse monoclonal

clone AOP-38, purified from hybridoma cell culture

别名:

Anti-Antioxidant-like Protein 1, Anti-PRDX3, Anti-Peroxiredoxin 3

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

偶联物

unconjugated

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

AOP-38, monoclonal

表单

buffered aqueous solution

分子量

antigen 25 kDa

种属反应性

monkey, chicken, canine, rat, human, mouse, hamster

浓度

~2 mg/mL

技术

indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using a whole extract of cultured human acute T cell leukemia, Jurkat, cells

同位素/亚型

IgG1

UniProt登记号

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... PRDX3(10935)
mouse ... Prdx3(11757)
rat ... Prdx3(64371)

一般描述

Anti-AOP1 (antioxidant-like protein 1) antibody, mouse monoclonal, (antioxidantlike protein 1) (mouse IgG1 isotype) is derived from the AOP-38 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of human Aop1, conjugated to keyhole limpet hemocyanine (KLH). Aop1 (antioxidant-like protein 1, also called MER5, M.W. 25 kDa).
Antioxidant-like Protein 1 (AOP1) belongs to a protein family that may regulate oxidative stress defense.

免疫原

synthetic peptide corresponding to C-terminal amino acids 236-256 of human Aop1, conjugated to KLH.

应用

AOP1 expression in cells isolated from human saliva was analyzed by western blot using mouse monoclonal anti-AOP1 antibody (A7674) at a 1:10000 dilution.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Mouse monoclonal anti-AOP1 antibody can be used for indirect ELISA and microarray applications.

生化/生理作用

AOP1 (antioxidant-like protein 1) is also proposed to be involved in the synthesis of glucosinolate. It defends oxidative stress and participates in proliferation and differentiation through antioxidant function and redox regulation. Monoclonal antibodies reacting specifically with Aop1 are useful tools for the determination of the functional activity of Aop1.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Jin-A Park et al.
Molecular medicine reports, 14(4), 3215-3219 (2016-08-12)
Antioxidant-like protein-1 (AOP-1) reduces the intracellular level of reactive oxygen species. In the present study, the age‑related change in AOP‑1 expression in the hippocampus among young, adult and aged gerbils was compared using western blot analysis and immunohistochemistry. The results demonstrated that
Mammalian antioxidant protein complements alkylhydroperoxide reductase (ahpC) mutation in Escherichia coli
Tsuji K, et al.
The Biochemical Journal, 307(2), 377-381 (1995)
Hongwei Xie et al.
Molecular & cellular proteomics : MCP, 7(3), 486-498 (2007-11-30)
Whole human saliva possesses tremendous potential in clinical diagnostics, particularly for conditions within the oral cavity such as oral cancer. Although many have studied the soluble fraction of whole saliva, few have taken advantage of the diagnostic potential of the
A Jäschke et al.
Journal of molecular biology, 277(4), 763-769 (1998-05-30)
Cyclophilins (CyPs) define a family of proteins binding to the immunosuppressive drug cyclosporin A (CsA). They are evolutionary highly conserved proteins being present in both pro- and eukaryotes and in different subcellular locations. CyPs possess enzymatic activity, namely peptidyl-prolyl cis-trans
Transcript residency on ribosomes reveals a key role for the A rabidopsis thaliana bundle sheath in sulfur and glucosinolate metabolism
Aubry S, et al.
The Plant Journal, 78(4), 659-673 (2014)

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