生物来源
mouse
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
AOP-38, monoclonal
表单
buffered aqueous solution
分子量
antigen 25 kDa
种属反应性
monkey, chicken, canine, rat, human, mouse, hamster
浓度
~2 mg/mL
技术
indirect ELISA: suitable
microarray: suitable
western blot: 0.25-0.5 μg/mL using a whole extract of cultured human acute T cell leukemia, Jurkat, cells
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... PRDX3(10935)
mouse ... Prdx3(11757)
rat ... Prdx3(64371)
一般描述
Anti-AOP1 (antioxidant-like protein 1) antibody, mouse monoclonal, (antioxidantlike protein 1) (mouse IgG1 isotype) is derived from the AOP-38 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of human Aop1, conjugated to keyhole limpet hemocyanine (KLH). Aop1 (antioxidant-like protein 1, also called MER5, M.W. 25 kDa).
Antioxidant-like Protein 1 (AOP1) belongs to a protein family that may regulate oxidative stress defense.
免疫原
synthetic peptide corresponding to C-terminal amino acids 236-256 of human Aop1, conjugated to KLH.
应用
AOP1 expression in cells isolated from human saliva was analyzed by western blot using mouse monoclonal anti-AOP1 antibody (A7674) at a 1:10000 dilution.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Mouse monoclonal anti-AOP1 antibody can be used for indirect ELISA and microarray applications.
生化/生理作用
AOP1 (antioxidant-like protein 1) is also proposed to be involved in the synthesis of glucosinolate. It defends oxidative stress and participates in proliferation and differentiation through antioxidant function and redox regulation. Monoclonal antibodies reacting specifically with Aop1 are useful tools for the determination of the functional activity of Aop1.
外形
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Jin-A Park et al.
Molecular medicine reports, 14(4), 3215-3219 (2016-08-12)
Antioxidant-like protein-1 (AOP-1) reduces the intracellular level of reactive oxygen species. In the present study, the age‑related change in AOP‑1 expression in the hippocampus among young, adult and aged gerbils was compared using western blot analysis and immunohistochemistry. The results demonstrated that
Mammalian antioxidant protein complements alkylhydroperoxide reductase (ahpC) mutation in Escherichia coli
Tsuji K, et al.
The Biochemical Journal, 307(2), 377-381 (1995)
Hongwei Xie et al.
Molecular & cellular proteomics : MCP, 7(3), 486-498 (2007-11-30)
Whole human saliva possesses tremendous potential in clinical diagnostics, particularly for conditions within the oral cavity such as oral cancer. Although many have studied the soluble fraction of whole saliva, few have taken advantage of the diagnostic potential of the
A Jäschke et al.
Journal of molecular biology, 277(4), 763-769 (1998-05-30)
Cyclophilins (CyPs) define a family of proteins binding to the immunosuppressive drug cyclosporin A (CsA). They are evolutionary highly conserved proteins being present in both pro- and eukaryotes and in different subcellular locations. CyPs possess enzymatic activity, namely peptidyl-prolyl cis-trans
Transcript residency on ribosomes reveals a key role for the A rabidopsis thaliana bundle sheath in sulfur and glucosinolate metabolism
Aubry S, et al.
The Plant Journal, 78(4), 659-673 (2014)
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