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Merck

41155

Sigma-Aldrich

Anti-Rabbit IgG−Abberior® CAGE 500 antibody produced in goat

for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

别名:

Abberior® CAGE 500P-Anti-Rabbit IgG antibody produced in goat

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.45

生物源

goat

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

rabbit

存貨情形

available only in USA and Canada

濃度

~1 mg/mL

螢光

λex 511 nm; λem 525 nm in PBS, pH 7.4 (photoactivated)

適合性

corresponds for SDS-PAGE

儲存溫度

−20°C

一般說明

Abberior CAGE 500 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye which fluoresces at 511 nm.
Abberior CAGE 500 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. It performs very well with Leica GSD microscope and other superresolution microscopes such as Nikon N-Storm, particularly as a 2 Color partner with CAGE 552 or FLIP 565.
Further, after photoactivation, Abberior CAGE 500 can be tracked to analyze molecular dynamics such as diffusion, flow directions and velocities (e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore).


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 511 (photoactivated, PBS pH 7.4), 299 (non-photoactivated, PBS pH 7.4)
Extinction Coefficient, εmax [M-1cm-1]: 50 000 (photoactivated, PBS pH 7.4)
Photoactivation Wavelength, λ [nm]: 360-440
Fluorescence Maximum, λem [nm]: 525 (photoactivated, PBS pH 7.4)
Recommended STED Wavelength, λ [nm]: 590-610
Fluorescence Quantum Yield, λ: 0,85 (photoactivated, PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (photoactivated, PBS pH 7.4)

適合性

Designed and tested for fluorescent super-resolution microscopy

分析報告

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

法律資訊

abberior is a registered trademark of Abberior GmbH

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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