349M-9

ZAP-70 (2F3.2) Mouse Monoclonal Antibody

• UNSPSC代码: 12352202
• NACRES: NA.41

属性

• 生物来源: mouse
• 偶联物: unconjugated
• 抗体形式: culture supernatant
• 抗体产品类型: primary antibodies
• 克隆: 2F3.2, monoclonal
• 描述: For In Vitro Diagnostic Use in Select Regions (See Chart)
• 表单: buffered aqueous solution
• 种属反应性: human
• 包装: vial of 0.1 mL concentrate (349M-94); vial of 0.5 mL concentrate (349M-95); bottle of 1.0 mL predilute (349M-97); vial of 1.0 mL concentrate (349M-96); bottle of 7.0 mL predilute (349M-98)
• 制造商/商品名称: Cell Marque^®
• 技术: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
• 同位素/亚型: IgG2a
• 控制: chronic lymphocytic leukemia
• 运输: wet ice
• 储存温度: 2-8°C
• 可视化: cytoplasmic
• 基因信息: human ... ZAP70(7535)

说明

一般描述

Zeta-associated protein-70 (ZAP-70), a member of the Syk family of tyrosine kinases, has an important role in T-cell receptor signaling, natural killer cell activation, and early B-cell development. ZAP-70 protein is not expressed in most normal mature B-cells, but is expressed in various B- and T-cell lymphomas. Attempts to implement ZAP-70 analysis in clinical flow cytometry laboratories have been fraught with difficulty; this approach is technically challenging and results often have been unreliable. Detection of ZAP-70 protein by immunohistochemistry using tissue sections of chronic lymphocytic leukemia/small lymphocyctic lymphoma (CLL/SLL) is an easy and inexpensive technique. The clinical course of CLL/SLL is extremely variable: in some patients it is aggressive and requires chemotherapy within a relatively short time of diagnosis; in others it is indolent and asymptomatic and may not require treatment for many years. It has recently been demonstrated that the mutational status of the immunoglobulin heavy-chain variable-region (IgVH) genes identifies 2 distinct groups of CLL/SLL: the patients with unmutated IgVH have an unfavorable clinical course, whereas those with mutated IgVH experience a favorable course. The reasons for the differences in the clinical course of CLL patients with mutated and unmutated IgVH genes are not clear. ZAP-70 expression is the best discriminator of mutated and unmutated IgVH genes, ZAP-70 positive (>20% tumor cells) being Ig-unmutated and ZAP-70 negative being Ig-mutated. Wiestner et al. demonstrated that ZAP-70 expression levels assessed by means of Western blotting, real-time RT-PCR and immunohistochemistry are closely correlated with IgVH mutational status.

质量

IVD

IVD

IVD

NA

联系

ZAP-70 Positive Control Slides, Product No. 349S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

外形

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

制备说明

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

其他说明

For Technical Service please contact: 800-665-7284 or email: [email protected]

法律信息

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 2
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable