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Merck

11191

Sigma-Aldrich

Histopaque®-1119

sterile-filtered, density: 1.119 g/mL

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

無菌

sterile-filtered

形狀

liquid

儲存期限

Expiry date on the label.

雜質

endotoxin, tested

密度

1.119 g/mL

應用

hematology
histology

儲存溫度

2-8°C

一般說明

Histopaque®-1119 是一种无菌、经过内毒素测试的即用型培养基。该溶液由聚蔗糖和泛影酸钠组成,经调整密度为 1.119 g/mL,pH 为 8.8–9.0。

應用

Histopaque®-1119适合与Histopaque-1077一起使用,因为它有助于从少量全血或骨髓中快速恢复活性单个核细胞和粒细胞。

特點和優勢

Histopaque®-1119 有许多优势,比如:
  • 可使活细胞极佳恢复
  • 允许选择分离不同种类的血细胞
  • 防止细胞变形
  • 保持细胞活力
  • 最小化外来细胞干扰
  • 各批次性能可重复
  • 经过过滤杀菌和内毒素检测
  • 在 2-8°C 避光保护条件下可稳定保存至少2年

原則

该过程涉及在 Histopaque-1119 上使等体积的 Histopaque-1077 分层,形成双梯度。将抗凝全血小心地铺在上层 Histopaque-1077 培养基上。离心后,红细胞被聚蔗糖聚集并因此迅速沉淀。粒细胞位于下部Histopaque-1077/1119 界面; 而在上部血浆/Histopaque-1077 界面处有淋巴细胞和其它单核细胞。在上部界面中发现的大多数外部血小板在洗涤步骤中通过低速离心去除。红细胞污染可以忽略不计。

法律資訊

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

产品编号
说明
价格

象形圖

Corrosion

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Circulation, 118(2), 140-148 (2008-06-25)
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Heterogeneity of gene expression and rarity of replication hamper molecular analysis of β-cell mass restoration in adult pancreas. Here, we show transcriptional dynamics in β-cell replication process by single-cell RNA sequencing of murine pancreas with or without partial pancreatectomy. We
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Group B Streptococcus (GBS) is major cause of invasive disease in newborn infants and the leading cause of neonatal meningitis. To gain access to the central nervous system (CNS), GBS must not only subvert host defenses in the bloodstream but
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Nature communications, 9(1), 1974-1974 (2018-05-19)
Glucose homeostasis depends critically on insulin that is secreted by pancreatic β-cells. Serum glucose, which is directly sensed by β-cells, stimulates depolarization- and Ca2+-dependent exocytosis of insulin granules. Here we show that pancreatic islets prominently express LRRC8A and LRRC8D, subunits

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实验方案

This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.

本疑难排查指南针对使用 Histopaque 时观察到的最常见错误来源提出解决方案,但这并不代表本指南是全面无遗的。

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