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Merck

10065

Sigma-Aldrich

α-淀粉酶 来源于米曲霉

powder, ~30 U/mg

别名:

1,4-α-D-葡聚糖-葡萄糖水解酶

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About This Item

EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
32160410
NACRES:
NA.54

形狀

powder

品質等級

比活性

~30 U/mg

分子量

Mr ~51000

儲存溫度

2-8°C

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相关类别

一般說明

微生物淀粉酶是胞外酶,可用于多种工业应用,如面包和麦芽糖浆生产、酱油和豆面酱发酵等。从米曲霉中分离的α-淀粉酶的分子量为51kDa(沉降和扩散)和49kDa(凝胶过滤)。

應用

α-淀粉酶已被用作
  • 琼脂平板和羧甲基纤维素清除试验的对照酶,用于筛选淀粉水解的纤维素酶活性,
  • 以便探讨小麦淀粉在冷冻面团中
  • 抑制金黄色葡萄球菌生物膜的作用。

生化/生理作用

米曲霉α-淀粉酶(Ao α-淀粉酶)酶催化可溶性淀粉和相关底物中α-1,4糖苷键的水解。这些底物被分解并释放较短的低聚糖和α-极限糊精。

單位定義

1个酶活力单位(U)相当于在pH6.0和25℃下每分钟释放1μmol麦芽糖所需的酶量(淀粉符合Zulkowsky,货号85642,作为底物)。

其他說明

用于测定食物中的脂肪;应用于糖苷键的(选择性)水解/缩合

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 4 Oral - Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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P C Sehnke et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(2), 765-770 (2001-01-10)
In higher plants the production of starch is orchestrated by chloroplast-localized biosynthetic enzymes, namely starch synthases, ADP-glucose pyrophosphorylase, and starch branching and debranching enzymes. Diurnal regulation of these enzymes, as well as starch-degrading enzymes, influences both the levels and composition
C E Daugherty et al.
Journal - Association of Official Analytical Chemists, 66(4), 927-932 (1983-07-01)
A chloroform-methanol extraction method (complete extraction of fat in 3 min) for determining fat in processed and prepared foods has been studied collaboratively. Fourteen collaborators reported single replicate fat results on 7 samples representative of various food types and 2
Mamadou Abdoulaye Konaré et al.
Plants (Basel, Switzerland), 12(2) (2023-01-22)
Currently, microencapsulation has become a viable method of nutrient delivery for the food industry. This work microencapsulated the bioactive compounds extracted from two neglected species (Balanites aegyptiaca and Ziziphus mauritiana) by freeze-drying. A combination of wall materials (whey protein and
S Wirsel et al.
Molecular microbiology, 3(1), 3-14 (1989-01-01)
We have cloned three genes (amy1, amy2 and amy3) encoding alpha-amylase in the filamentous fungus Aspergillus oryzae. The established overall sequences have a very high degree of homology, showing divergences mainly in the 3'-untranslated regions. The positions and the sequences
Han Tao et al.
Food chemistry, 265, 18-22 (2018-06-10)
Properties of starches isolated from soft and hard wheat dough after freezing/thawing (F/T) treatment were investigated. Significance of results was observed between isolated hard wheat starch (HWS) and soft wheat starch (SWS), but both cultivars showed an increase in the

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