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產品線
BioReagent
形狀
powder
製造商／商標名
ATTO-TEC GmbH
溶解度
DMF: soluble
DMSO: soluble
acetonitrile: soluble
螢光
λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
適合性
suitable for fluorescence
suitable for gel electrophoresis
儲存溫度
−20°C
應用
Atto 655 is a fluorescent label with high molecular absorption (125.000) and quantum yield (0.30), as well as sufficient stoke′s shift (excitation maximum 663 nm, emission maximum 684 nm). There is very little cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Atto 655 has been coupled to streptavidin to enable various types of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays.
分析報告
free of unconjugated dye
法律資訊
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
Simple buffers for 3D STORM microscopy.
van de Linde, S.; Kasper, R.; Heilemann, M.; Sauer, M.
Applied Physics B: Lasers and Optics, 93(4), 725-731 (2008)
Triple-Color Super-Resolution Imaging of Live Cells. Resolving Submicroscopic Receptor Organization in the Plasma Membrane.
Wilmes, S.; Staufenbiel, M.; Lisse, D.; Richter, Ch. P.; Beutel, O.; Busch, K. B. et al.
Angewandte Chemie (International Edition in English), 124(20), 4952-4955 (2012)
Nicolas Olivier et al.
Biomedical optics express, 4(6), 885-899 (2013-06-14)
3D STORM is one of the leading methods for super-resolution imaging, with resolution down to 10 nm in the lateral direction, and 30-50 nm in the axial direction. However, there is one important requirement to perform this type of imaging:
Dynamic Superresolution Imaging of Endogenous Proteins on Living Cells at Ultra-High Density.
Giannone, G.; et al.
Biophysical Journal, 99(4), 1303-1310 (2010)
商品
Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.
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