生物源
goat
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
物種活性
rabbit
濃度
~1 mg/mL
螢光
λex 511 nm; λem 530 nm in PBS, pH 7.4
儲存溫度
−20°C
一般說明
Abberior STAR 512 was developed for STED and confocal microscopy in the green spectral region. Abberior STAR 512 is highly photostable and bright green fluorescent dye. The dye works extremely well with Abberior Instruments Expert Line Microscopes and Leica STED microscopes. It can be very effectively excited with excitation lasers at 488 nm or 514 nm. Abberior STAR 512 can substitute dyes like Oregon Green 514, Atto™ 514 or Alexa Fluor™ 514. For STED microscopy, Abberior STAR 512 can be most efficiently used with STED Laser wavelengths between 590 - 610 nm. Best results are obtained with freshly prepared samples.
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 511 (PBS pH 7.4), 512 (H2O), 517 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 85 000 (PBS pH 7.4), 92 000 (H2O), 95 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,24 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,07 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 530 (PBS pH 7.4), 530 (H2O), 533 (MeOH)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,82 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (PBS pH 7.4)
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 511 (PBS pH 7.4), 512 (H2O), 517 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 85 000 (PBS pH 7.4), 92 000 (H2O), 95 000 (MeOH)
Correction Factor, CF260 = ε260/εmax: 0,24 (PBS pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0,07 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 530 (PBS pH 7.4), 530 (H2O), 533 (MeOH)
Recommended STED Wavelength, λ [nm]: 590 - 610
Fluorescence Quantum Yield, λ: 0,82 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (PBS pH 7.4)
適合性
Designed and tested for fluorescent super-resolution microscopy
分析報告
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
法律資訊
ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
S. M. Polyakova, et al.,
European Journal of Organic Chemistry, 30, 5162-5177 (2009)
Gyuzel Yu Mitronova et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 16(15), 4477-4488 (2010-03-24)
New photostable rhodamine dyes represented by the compounds 1 a-r and 3-5 are proposed as efficient fluorescent markers with unique combination of structural features. Unlike rhodamines with monoalkylated nitrogen atoms, N',N-bis(2,2,2-trifluoroethyl) derivatives 1 e, 1 i, 1 j, 3-H and 5 were found to
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