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Merck

93321

Sigma-Aldrich

TRIS 甘氨酸缓冲液 溶液

BioUltra, 10× concentrate

别名:

印迹缓冲剂 溶液

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About This Item

分類程式碼代碼:
41105319
NACRES:
NA.25

產品線

BioUltra

品質等級

形狀

liquid

品質

10× concentrate

成份

glycine, 1.92 M
TRIS, 0.25 M

雜質

insoluble matter, passes filter test
proteases, none detected

燃燒殘留物

≤0.2%

pH值

8.5-8.7

負離子痕跡

sulfate (SO42-): ≤50 mg/kg

正離子痕跡

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
NH4+: ≤200 mg/kg
Na: ≤500 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

&lambda ;

neat

紫外吸收

λ: 260 nm Amax: 0.1
λ: 280 nm Amax: 0.1

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應用

Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. TRIS Glycine buffer solution, 10X concentrate is diluted to a working concentration of 1X and pH adjusted as required. TRIS Glycine buffer (TGB) is frequently used in gel electrophoresis and ion-exchange chromatography applications.

其他說明

从聚丙烯酰胺凝胶到硝化纤维片的蛋白质电泳转移:步骤和一些应用

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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Hongshan Liu et al.
Molecular vision, 15, 505-517 (2009-03-06)
To develop a new method of whole mount immunostaining that improves the penetration of staining reagents into the cornea and decreases non-specific binding and background. Adult mouse corneas were fixed overnight in 4% paraformaldehyde or a mixture of 4% paraformaldehyde
Guo-Min Tan et al.
Journal of chromatography. A, 1098(1-2), 131-137 (2005-11-30)
Ion-exchange electrochromatography with an oscillatory electric field perpendicular to mobile-phase flow driven by pressure (pIEEC) was developed with a column design of rectangle cross-section. The effect of electric field strength on the dynamic binding capacity (DBC) was examined by frontal
Zhixin Wang et al.
Electrophoresis, 29(22), 4454-4462 (2008-11-28)
Multiple labeling of nucleic acids by intercalative dyes is a promising method for ultrasensitive nucleic acid assays. The properties of the fast dissociation and instability of dye-DNA complexes may prevent from their wide applications in CE-LIF nucleic acid analysis. Here
E R Frears et al.
Glycoconjugate journal, 16(6), 283-290 (1999-12-01)
IgG carries bi-antennary N-linked glycans which differ in degrees of galactosylation, core fucosylation and bisecting N-acetyl glucosamine. The majority of these are non-sialyated closely related neutral structures which can be resolved by HPLC analysis, but which are difficult to separate
H Towbin et al.
Proceedings of the National Academy of Sciences of the United States of America, 76(9), 4350-4354 (1979-09-01)
A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quantitative transfer of ribosomal proteins from gels containing urea. For sodium dodecyl sulfate gels, the original band pattern was

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