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Merck

10197734001

Roche

谷氨酸脱氢酶(GlDH)

from beef liver

别名:

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About This Item

分類程式碼代碼:
12352204

生物源

bovine liver

品質等級

形狀

lyophilized

比活性

10 U/mg
~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)

包裝

pkg of 3,000 U

製造商/商標名

Roche

濃度

≥10-20 % (w/w)

技術

activity assay: suitable

顏色

white

最適pH

8

溶解度

water: 20 mg/mL, colorless

適合性

suitable for UV spectrophotometry and general use

UniProt登錄號

應用

life science and biopharma

異物活動

ADH <0.00500%
LDH <0.00500%
MDH <0.00500%

運輸包裝

wet ice

儲存溫度

2-8°C

基因資訊

bovine ... GLUD1(281785)

一般說明

GDH(谷氨酸脱氢酶)是含有449个残基的六聚体,其位于线粒体中。它可充当氨基酸氧化和尿素生成之间的分支酶。L-谷氨酸:NAD(P)+氧化还原酶参与脱氨。
Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.

應用

在葡萄酒酵母发酵过程中,谷氨酸脱氢酶用于通过酶分析测定残留铵。它作为NAD(P)和NAD(P)H 辅助因子循环系统的组分。

品質

污染物:分别含有<0.005%的ADH、LDH和MDH

序列

GIDH(310,000至350,000)的最小活性结构是一个55,400D的六聚体亚基。该六聚体能够可逆地自组装形成具有多达八个六聚体(2,200 kD)的聚合物。

其他說明

仅用于生命科学研究。不可用于诊断。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

does not flash

閃點(°C)

does not flash


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Disruption of the cell wall integrity gene ECM33 results in improved fermentation by wine yeast
Jin Zhang
Metabolic engineering (2018)
T J Stillman et al.
Journal of molecular biology, 234(4), 1131-1139 (1993-12-20)
We have solved the structure of the binary complex of the glutamate dehydrogenase from Clostridium symbiosum with glutamate to 1.9 A resolution. In this complex, the glutamate side-chain lies in a pocket on the enzyme surface and a key determinant
G Hughes et al.
Analytica chimica acta, 933, 82-88 (2016-08-09)
A reagentless glutamate biosensor was applied to the determination of glutamate released from liver hepatocellular carcinoma cells (HepG2) in response to toxic challenge from various concentrations of paracetamol. A screen printed carbon electrode (SPCE) containing the electrocatalyst Meldola's Blue (MB-SPCE)
P J O'Brien et al.
Laboratory animals, 36(3), 313-321 (2002-07-30)
In a recent study in rats, alanine aminotransferase (ALT), the preferred plasma biomarker of hepatocellular injury in rats, was ineffective at detecting marked hepatic necrosis produced by acetaminophen (Human and Experimental Toxicology 19, 277-83, 2000). In contrast, glutamate dehydrogenase (GLDH)
Po Chen et al.
Fly, 14(1-4), 3-9 (2020-07-04)
Glutamine: fructose-6-phosphate amidotransferase (GFAT) enzymes catalyse the first committed step of the hexosamine biosynthesis pathway (HBP) using glutamine and fructose-6-phosphate to form glucosamine-6-phosphate (GlcN6P). Numerous species (e.g. mouse, rat, zebrafish, chicken) including humans and Drosophila encode two broadly expressed copies

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