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形狀
liquid
品質等級
純化經由
affinity chromatography
包裝
pkg of 100 μg
技術
affinity binding assay: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
mass spectrometry (MS): suitable
proximity ligation assay: suitable
儲存溫度
-65 to -96°C
一般說明
Protein A Turbo is a recombinantly expressed Protein A-TurboID fusion protein with the capability to bind IgG through the Protein A region, and enzymatically biotinylate proximal proteins non-specifically with the TurboID region.
特異性
Protein A strongly binds multiple Immunoglobulin G subclasses (IgG1, IgG2, IgG4), but does not bind IgG3. This probe is not compatible with primary antibodies that are IgG3.
應用
- Proximity protein labeling
- Cell Biology
- Histology
- Fixed cell microscopy
- Tissue staining
- Immunofluorescence
- Liquid Chromatography
- Mass Spectrometry
- Immunoprecipitation
外觀
Purified protein in 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 150 mM imidazole with protease inhibitor cocktail.
重構
0.7 mg/mL
儲存和穩定性
Store at -65°C to -96°C.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
訊號詞
Danger
危險聲明
危險分類
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
儲存類別代碼
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Irene Santos-Barriopedro et al.
Nature communications, 12(1), 5015-5015 (2021-08-20)
Proximity biotinylation workflows typically require CRISPR-based genetic manipulation of target cells. To overcome this bottleneck, we fused the TurboID proximity biotinylation enzyme to Protein A. Upon target cell permeabilization, the ProtA-Turbo enzyme can be targeted to proteins or post-translational modifications
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