抗体形式
purified antibody
质量水平
克隆
100, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human
请勿与下列物质发生反应
mouse
同位素/亚型
IgG1
一般描述
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-AG14 plasmacytoma cells (see application references). Recognizes the ~24-26 kDa bcl-2 protein.
Recognizes the ~24-26 kDa Bcl-2 protein in MCF-7 cells, tonsil, and lymph node tissue.
The bcl-2 proto-oncogene is encoded by a gene 230 kb in size that gives rise to a ~24-26 kDa protein which has been localized to the mitochondrial membrane. Although some data exists for implicating bcl-2 as a novel GTP binding protein, the major function of bcl-2 appears to be an inhibitor of apoptosis or "programmed cell death". It has now been well documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore constitutively activated in B cell malignancies (i.e. follicular lymphoma and chronic lymphocytic leukemia). In addition, bcl-2 has also been shown to protect mammalian cells from sodium azide, colchicine, DNA, RNA, and protein-synthesis inhibitors, as well as steroids, heat shock and irradiation.
免疫原
a synthetic peptide (GAAPAPGIFSSQPG) corresponding to amino acids 41-54 of human Bcl-2
应用
Frozen Sections (1-5 μg/ml)
Immunoblotting (1-5 μg/ml)
Paraffin Sections (1-10 μg/ml, see comments)
Immunoblotting (1-5 μg/ml)
Paraffin Sections (1-10 μg/ml, see comments)
外形
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
分析说明
Positive Control
MCF7 cells or tonsil or lymph node tissue
MCF7 cells or tonsil or lymph node tissue
其他说明
Generally, no pre-treatment of paraffin sections is needed, but treating with heat may improve staining in some tissues. Antibody should be titrated for optimal results in individual sample types.
J C Reed et al.
Molecular and cellular biology, 10(8), 4370-4374 (1990-08-01)
The BCL2 (B cell lymphoma/leukemia-2) and C-HA-RAS oncogenes encode membrane-associated proteins of 26 and 21 kilodaltons, respectively. Although RAS proteins have long been known for their ability to bind and hydrolyze GTP, recent investigations suggest that BCL2 encodes a novel
J C Reed et al.
Experimental cell research, 195(2), 277-283 (1991-08-01)
The BCL2 (B cell lymphoma/leukemia-2) proto-oncogene encodes a 26-kDa protein that has been localized to the inner mitochondrial membrane and that has been shown to enhance the survival of some types of hematopoietic cells. Here we show that NIH3T3 fibroblasts
D L Vaux
Proceedings of the National Academy of Sciences of the United States of America, 90(3), 786-789 (1993-02-01)
Cell death is a normal physiological process. Morphological studies have shown that cells that die by physiological mechanisms often undergo characteristic changes termed "apoptosis" or "programmed cell death." Recent work has begun to unravel the molecular mechanisms of these deaths
D M Hockenbery et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(16), 6961-6965 (1991-08-15)
The BCL2 protooncogene encodes an inner mitochondrial membrane protein that blocks programmed cell death. BCL2 was isolated from the chromosomal breakpoint of follicular B-cell lymphoma. Transgenic mice that overexpress BCL2 display extended survival of resting B cells. In this study
G Nunez et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(12), 4589-4593 (1989-06-01)
Human follicular B-cell lymphomas possess a t(14;18) that translocates a putative protooncogene, BCL2, into the immunoglobulin heavy chain locus. The normal BCL2 gene is quiescent in resting B cells, expressed in proliferating, but down-regulated in differentiated B cells. Inappropriately high
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