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MT17MAG47K-PX25

Millipore

MILLIPLEX®小鼠TH17磁珠板 - 预混25重 - 免疫学多重分析

Simultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

别名:

Mouse TH17 Panel, TH17 Magnetic Bead Panel

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.47

品質等級

物種活性

mouse

製造商/商標名

Milliplex®

assay range

accuracy: 85-110%
linearity: 111.0%
(1:04)

linearity: 113.1%
(1:08)

standard curve range: 1.5-1,500 pg/mL
(IL-4)

standard curve range: 10-10,000 pg/mL
(IL-17F)

standard curve range: 127-130,000 pg/mL
(IL-28B)

standard curve range: 15-15,000 pg/mL
(IL-1β)

standard curve range: 2.4-2,500 pg/mL
(IL-22)

standard curve range: 20-20,000 pg/mL
(IL-10, IL-12(p70), & IL-21)

standard curve range: 3.4-3,500 pg/mL
(TNFα)

standard curve range: 34-35,000 pg/mL
(GM-CSF & IL-15)

standard curve range: 342-350,000 pg/mL
(IL-23)

standard curve range: 39-40,000 pg/mL
(IL-13 & IL-17A)

standard curve range: 4.9-5,000 pg/mL
(IL-5)

standard curve range: 488-500,000 pg/mL
(TNFβ)

standard curve range: 49-50,000 pg/mL
(CD40L IL-31 & MIP-3α)

standard curve range: 586-600,000 pg/mL
(IL-17E/ IL-25)

standard curve range: 6.9-6,000 pg/mL
(IL-2)

standard curve range: 7.8-8,000 pg/mL
(IFNγ & IL-6)

standard curve range: 78-80,000 pg/mL
(IL-33)

standard curve range: 879-900,000 pg/mL
(IL-27)

技術

multiplexing: suitable

相容性

configured for Premixed

檢測方法

fluorometric (Luminex xMAP)

運輸包裝

wet ice

一般說明

CD4 T-辅助细胞是适应性免疫的主要参与者。基于它们的转录因子和分泌的细胞因子的表达谱,这些细胞被分为主要的亚类Th1,Th2,Th17和调节性(Treg)T细胞 。由IL-12诱导并由IFNγ增强的Th1细胞分泌激活巨噬细胞的IFNγ。在IL-4诱导下,Th2细胞主要产生IL-4,IL-5,IL-13,IL-25和IL-17E,在过敏中起作用。由TGFβ诱导的Treg细胞产生抗炎细胞因子IL-10和TGFβ以及在控制T细胞反应中起作用,从而防止自身免疫反应。Th17细胞是最近发现的以IL-17产生为特征的亚类,在人中由TGFβ诱导,与IL-23和IL-1β结合,在小鼠中由TGFβ,IL-6和IL-21结合诱导。活化的Th17细胞分泌IL-17A、IL-17F、IL-21、IL-22和TNFα促进组织炎症。

Th17细胞参与细胞外细菌和真菌的清除。它们在肠固有层中含量丰富,作为抵抗入侵病原体的屏障。过量的Th17细胞与几种自身免疫性疾病的发病机理有关,包括多发性硬化、银屑病、青少年糖尿病、类风湿关节炎、克罗恩病和自身免疫葡萄膜炎。此外,Th17细胞在肿瘤的发展,进展和转移中起着重要作用,可能在促进和抑制肿瘤的生长中都起着重要作用。

MILLIPLEX®小鼠Th17珠组套使您能够专注于调节CD4 T辅助细胞细胞因子的治疗潜力。结合微珠格式的Luminex ® xMAP®平台,您可以获得理想的速度和灵敏度的优势,允许同时对数十种分析物进行定量多重检测,从而可以显著提高生产力。

组套类型:细胞因子/趋化因子

特異性

交叉反应性
抗体与样品组中的任何其他分析物之间没有交叉反应性或交叉反应性最小(请参见测定方案中的详细信息)。
本组套中抗体与任何其他分析物之间的交叉反应性不可检出或可忽略不计。

應用

  • 分析物:CD40配体、GM-CSF、IFN-γ、IL-1β、IL-2、IL-4、IL-5、IL-6、IL-10、IL-12(p70)、IL-13、IL-15、IL-17A、IL-17E/IL-25、IL-17F、IL-21、IL-22、IL-23、IL-27、IL-28B、IL-31、IL-33、MIP-3α/CCL20、TNF-α、TNFβ
  • 建议的样品类型:血清、血浆或组织/细胞裂解物和培养物上清液
  • 建议的样品稀释度:纯品
  • 测定运行时间:过夜或一天
  • 研究类别:炎症&免疫学

儲存和穩定性

试剂盒组件的建议储存温度为2 - 8°C。

其他說明

灵敏度:有关单个生物标志物的灵敏度,请参阅试剂盒方案。

法律資訊

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

標靶器官

Respiratory Tract

儲存類別代碼

10 - Combustible liquids


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