MABT1565

Anti-Desmin Antibody, clone DE-U-10

• 别名: CMD1I, CSM1, CSM2
• UNSPSC代码: 12352203
• NACRES: NA.43

属性

• 生物来源: mouse
• 质量水平: 200
• 抗体形式: purified antibody
• 抗体产品类型: primary antibodies
• 克隆: DE-U-10, monoclonal
• 分子量: calculated mol wt 53.6 kDa; observed mol wt ~16 kDa
• 纯化方式: using protein G
• 种属反应性: hamster, human, rat, mouse
• 种属反应性（根据同源性预测）: porcine
• 包装: antibody small pack of 100
• 技术: ELISA: suitable; immunofluorescence: suitable; western blot: suitable
• 同位素/亚型: IgG1κ
• 表位序列: C-terminal half
• Protein ID登记号: NP_001001535
• UniProt登记号: P02540
• 储存温度: 2-8°C
• 基因信息: Porcine ... DES(396725)

说明

特异性

Clone DE-U-10 is a mouse monoclonal antibody that detects Desmin. It targets an epitope within 48 amino acids from the C-terminal half of the protein.

免疫原

Purified full length porcine Desmin.

应用

Quality Control Testing

Evaluated by Western Blotting in mouse skeletal muscle tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Desmin in mouse skeletal muscle tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Desmin in human skeletal muscle tissue extracts.

ELISA Analysis: A representative lot detected Desmin in ELISA applications (Debus, E., et al. (1983). EMBO J. 2(12):2305-12).

Western Blotting Analysis: A representative lot detected Desmin in Western Blotting applications (Debus, E., et al. (1983). EMBO J. 2(12):2305-12).

Immunofluorescence Analysis: A representative lot detected Desmin in Immunofluorescence applications (Debus, E., et al. (1983). EMBO J. 2(12):2305-12).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

目标描述

Desmin (UniProt: P02540) is encoded by the DES gene (Gene ID: 396725) in porcine. Desmin is a type III intermediate filament protein that is essential for maintaining the structural and functional integrity of muscle cells as part of the cytoskeletal network. It provides mechanical support to the muscle cells and facilitates the organization of contractile proteins within the cell. Additionally, it is involved in various cellular processes, including cell signaling, maintenance of cell shape, and intracellular transport. It is particularly abundant in cardiac, skeletal, and smooth muscle tissues. It is also enriched at postsynaptic membranes of neuromuscular junctions (NMJs). Mutations in the gene encoding Desmin have been associated with hereditary and sporadic myopathies, cardiomyopathies and myasthenic syndrome. (Ref.: Eiber, N., et al., (2020). Front. Mol. Neurosci. 13; Article 567084).

外形

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

重悬

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommended storage: +2°C to +8°C.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable