MABS421

Anti-DKK3, clone 4G7 Antibody

ascites fluid, clone 4G7, from mouse

• 别名: Dickkopf-related protein 3, Dickkopf-3, Dkk-3, hDkk-3
• UNSPSC代码: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物来源: mouse
• 质量水平: 100
• 抗体形式: ascites fluid
• 抗体产品类型: primary antibodies
• 克隆: 4G7, monoclonal
• 种属反应性: human
• 技术: flow cytometry: suitable; western blot: suitable
• 同位素/亚型: IgG1
• UniProt登记号: Q9UBP4
• 运输: wet ice
• 靶向翻译后修饰: unmodified
• 基因信息: human ... DKK3(27122)

说明

一般描述

Dickkopf-related protein 3, also known as Dickkopf-3, or Dkk-3, or hDkk-3, and encoded by the gene DKK3/ REIC is a member of the dickkopf family. DKK3 is a 350 amino acid secreted glycoprotein that is composed of an N-terminal signal peptide and two conserved cysteine-rich domains, which are separated by a 12 amino acid linker region. This secreted protein is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.

免疫原

Purified recombinant fragment of human DKK3 expressed in E. Coli

应用

Anti-DKK3, clone 4G7 Antibody is an antibody against DKK3 for use in western blotting & flow cytometry.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected DKK3 in MCF-7 cells.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

质量

Evaluated by Western Blotting in HEK293, MCF-7 and HL7702 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected DKK3 in HEK293, MCF-7 and HL7702 cell lysates.

目标描述

~44 kDa observed. Uncharacterized bands may be observed in some lysate(s).

外形

Unpurified

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: nwg
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable