MABS1708

Anti-SAV1 Antibody, clone 3A1.1

clone 3A1.1, from mouse

• 别名: Protein salvador homolog 1, 45 kDa WW domain protein, hWW45
• UNSPSC代码: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物来源: mouse
• 质量水平: 100
• 抗体形式: purified antibody
• 抗体产品类型: primary antibodies
• 克隆: 3A1.1, monoclonal
• 种属反应性: human
• 包装: antibody small pack of 25 μg
• 技术: immunohistochemistry: suitable (paraffin); western blot: suitable
• 同位素/亚型: IgG2aκ
• NCBI登记号: NP_068590
• UniProt登记号: Q9H4B6
• 运输: ambient
• 靶向翻译后修饰: unmodified
• 基因信息: human ... SAV1(60485)

说明

一般描述

Protein salvador homolog 1 (UniProt: Q9H4B6; also known as 45 kDa WW domain protein, hWW45) is encoded by the SAV1 (also known as WW45) gene (Gene ID: 60485) in human. SAV1, a homodimeric protein is ubiquitously expressed in adult tissues with highest expression observed in the pancreas, aorta, and interventricular septum and lowest expression in skeletal muscle. SAV1 protein contains two WW domains (aa 199-232 and 234-267) and one SARAH domain (aa 321-368). SAV1 is phosphorylated by STK3/MST2 and STK4/MST1, but phosphorylation is not required for its stability. However, it increases the number of protein binding sites on the scaffold molecule. SAV1 is also required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. SAV1 regulates their activity in the Hippo signaling pathway, which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. STK3/MST2 and STK4/MST1- SAV1 complex is also shown to phosphorylate and activate LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 inhibits its translocation into the nucleus.

特异性

Clone 3A1.1 detects Protein salvador homolog 1 in human cells It targets an epitope with in 101 amino acids from the C-terminal half.

免疫原

GST/His-tagged recombinant fragment corresponding to 101 amino acids from the C-terminal half of human Protein salvador homolog 1 (SAV1).

应用

Anti-SAV1, clone 3A1.1, Cat. No. MABS1708, is a mouse monoclonal antibody that detects Protein salvador homolog 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SAV1 in 10 µg of OVCAR3 cell lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected SAV1 in human kidney and human pancreas tissues.

质量

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SAV1 in 10 µg of HCT116 cell lysate.

目标描述

~45 kDa observed; 44.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable