生物源
hamster
品質等級
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
H24G11, monoclonal
分子量
calculated mol wt 70.33 kDa
observed mol wt ~85 kDa
純化經由
using protein G
物種活性
mouse
包裝
antibody small pack of 100 μL
技術
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgGκ
表位序列
Unknown
Protein ID登錄號
UniProt登錄號
運輸包裝
2-8°C
目標翻譯後修改
unmodified
基因資訊
mouse ... Crtac1(72832)
一般說明
Cartilage acidic protein 1 (UniProt: Q8R555; also known as 68 kDa chondrocyte-expressed protein, CEP-68, ASPIC, Protein CRTAC1-B, Lateral olfactory tract usher substance, LOTUS) is encoded by the Crtac1 (also known as Aspic1, Cep68) gene (Gene ID: 72832) in murine species. LOTUS is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to generate the mature form (aa 29-646). It is a membrane-bound and/or secreted protein that serves as an endogenous Nogo receptor-1 (NgR1) antagonist. It is shown to contribute to the formation of the lateral olfactory tract (LOT) axonal bundle. Endogenous Nogo-A signaling through NgR1 is shown to induce axonal branching. Developmental up-regulation of Nogo-A promotes collateral formation of the LOT during the late development period. It has been reported that axon growth inhibitors, which include Nogo proteins, myelin-associated glycoprotein (MAG), oligodendrocyte myelin glycoprotein (OMgp), and B lymphocyte stimulator (BLyS), bind to NgR1 and restrict functional recovery after damage to the adult central nervous system. LOTUS blocks the binding of these ligands to NgR1 and suppresses growth cone collapse. LOTUS knockout mice show significantly increased collateral branches of axons in the LOT. On the other hand, this branching is diminished in NgR1 knockout mice. A simultaneous loss of LOTUS and NgR1 is reported to rescue the abnormal increase in axonal branches. (Ref.: Iketani, M., et al. (2016). Sci. Rep. 6; 39586; Kurihara, Y., et al. (2014). Mol. Cell. Neurosci. 61; 211-218).
特異性
Clone H24G11 is a hamster monoclonal antibody that detects murine cartilage acidic protein 1B (LOTUS).
免疫原
Homogenate of protein extract from developing lateral olfactory tract and the surrounding tissues of mouse.
應用
Quality Control Testing
Evaluated by Western Blotting in Mouse E14 brain tissue lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected LOTUS in Mouse E14 brain tissue lysates.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected LOTUS in Immunocytochemistry applications (Kurihara, Y., et al. (2014). Mol Cell Neurosci. 61:211-8; Sato, Y., et al. (2011). Science. 333(6043):769-73).
Immunohistochemistry Applications: A 1:50 dilutions from a representative lot detected LOTUS in E14 embryonic mouse tissue sections.
Western Blotting Analysis: A representative lot detected LOTUS in Western Blotting applications (Iketani, M., et al. (2016). Sci Rep. 6:39586).
Immunohistochemistry Applications: A representative lot detected LOTUS in Immunohistochemistry applications ( Sato, Y., et al. (2011). Science. 333(6043):769-73; Iketani, M., et al. (2016). Sci Rep. 6:39586).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Mouse E14 brain tissue lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected LOTUS in Mouse E14 brain tissue lysates.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected LOTUS in Immunocytochemistry applications (Kurihara, Y., et al. (2014). Mol Cell Neurosci. 61:211-8; Sato, Y., et al. (2011). Science. 333(6043):769-73).
Immunohistochemistry Applications: A 1:50 dilutions from a representative lot detected LOTUS in E14 embryonic mouse tissue sections.
Western Blotting Analysis: A representative lot detected LOTUS in Western Blotting applications (Iketani, M., et al. (2016). Sci Rep. 6:39586).
Immunohistochemistry Applications: A representative lot detected LOTUS in Immunohistochemistry applications ( Sato, Y., et al. (2011). Science. 333(6043):769-73; Iketani, M., et al. (2016). Sci Rep. 6:39586).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-LOTUS, clone H24G11, Cat. No. MABN2614, is a hamster monoclonal antibody that detects Cartilage acidic protein 1 (LOTUS) and is tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
外觀
Purified hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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