生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
IF15-E2, monoclonal
分子量
calculated mol wt 64.06 kDa
observed mol wt ~58 kDa
种属反应性
human, mouse
包装
antibody small pack of 100 μL
技术
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同位素/亚型
IgG
UniProt登记号
运输
dry ice
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
SHC-transforming protein 3 (UniProt: Q92529; also known as Neuronal Shc, N-Shc, Protein Rai, SHC-transforming protein C, Src homology 2 domain-containing-transforming protein C3, SH2 domain protein C3, Rai) is encoded by the SHC3 (also known as NSHC, SHCC) gene (Gene ID:53358) in human. Rai protein is mainly expressed in the brain and its higher expression is observed in cerebral cortex, frontal and temporal lobes, hippocampus, and occipital pole. Its expression is also reported in the enteric glial cells in the human gut. It serves as a signaling adapter and is involved in the signal transduction pathways of neurotrophin-activated Trk receptors in cortical neurons. Rai also serves as a physiological substrate of the receptor tyrosine kinase Ret and increases Ret-dependent survival via PI3 kinase/Akt pathway activation. It also potentiates MAP kinase signaling. Rai activation also plays a role in long-term potential and is neuroprotective against certain ischemia and oxidative stress. Evolutionarily it correlates well with vertebrate brain development. Its reduced levels are observed in brain of Alzheimer s disease subjects. Two isoforms of Rai have been described (p64 and p52) that are produced by alternative splicing. (Ref.: Triaca, V., et al. (2018). Aging. 10(1); 5-6; Sagi, O., et al. (2015). Ageing Res. Rev. 19; 34-42; Villanacci, V., et al. (2008). Neurogastroenterol. Motil. 20(3); 206-212).
特异性
Clone IF15-E2 is a mouse monoclonal antibody that detects SHC-transforming protein 3/RAI. It targets an epitope within the CH1 domain from the C-terminal half.
免疫原
Recombinant fragment corresponding to 164 amino acids CH1 domain of human SHC-transforming protein 3 (Rai).
应用
Quality Control Testing
Evaluated by Western Blotting in Human brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Rai in Human brain tissue lysate.
Tested Applications
Immunohistochemistry Applications: A representative lot detected Rai in Immunohistochemistry applications (Villanacci, V., et. al. (2008). Neurogastroenterol Motil. 20(3):206-12).
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rai in U2OS cells.
Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected Rai in human esophagus, stomach, and colon tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Rai in Human brain tissue lysate.
Tested Applications
Immunohistochemistry Applications: A representative lot detected Rai in Immunohistochemistry applications (Villanacci, V., et. al. (2008). Neurogastroenterol Motil. 20(3):206-12).
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rai in U2OS cells.
Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected Rai in human esophagus, stomach, and colon tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Rai, clone IF15, Cat. No. MABN2286, is a mouse monoclonal antibody that detects SHC-transforming protein 3 (Rai) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
外形
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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