生物源
hamster (Armenian)
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
9E9, monoclonal
物種活性
mouse
技術
flow cytometry: suitable
neutralization: suitable
同型
IgG
NCBI登錄號
UniProt登錄號
運輸包裝
ambient
目標翻譯後修改
unmodified
基因資訊
mouse ... Fcgr4(246256)
一般說明
Transmembrane receptor CD16-2 (UniProt: Q8R477; also known as CD16-2, Fcgr4, FcgRIV, Fc gammaRIV, FcgammaRIV, Fcgr3a, CD16-2, Transmembrane receptor CD16-2) is encoded by the Fcgr4 (also known as Fcgr3a, Fcrl3) gene (Gene ID: 245256) in murine species. Murine species express three classes of activating IgG Fc receptors, the high affinity receptor FcgRI, the low affinity receptor FcgRIII, and the FcgRIV or CD16-2. CD16-2, a novel mouse receptor that displays significant homology to CD16/FcgRIII. It is a receptor for soluble IgG2a and 2b complexes, but not necessarily for cellular bound IgG2a. CD16-2 is expressed in peripheral blood leukocytes and in spleen, thymus, colon, and intestine. Similar to other activating receptors, CD16-2 possesses a charged residue within its transmembrane domain that is necessary for association with an ITAM (immunoreceptor tyrosine-based activation motif) bearing accessory protein. Both CD16-2 (FcgRIV) and FcgRIII are considered to be essential for mediating type II and type III autoimmune responses via FcRc-LAT-dependent generation of C5a. However, FcgRIII-deficient mice display compensatory enhanced CD16-2 expression that offers protection from lung inflammation and exhibit reduced sensitivity to IgG2a/b-mediated hemolytic anemia. Ref.: Syed, S.N., et al (2009). Eur. J. Immunol. 39(12): 3343-3356; Mechetina, L.V., et al. (2002). Immunogenetics 54(7): 463-468.
The previously assigned protein identifier Q8R477 has been merged into A0A0B4J1G0. Full details can be found on the UniProt database.
The previously assigned protein identifier Q8R477 has been merged into A0A0B4J1G0. Full details can be found on the UniProt database.
特異性
Clone 9E9 immunostained murine macrophages, neutrophils, dendritic cells, but not FcgRIII-expressing NK cells. Clone 9E9 also stained a FcgRIV-negative, B220-positive peripheral blood cell population (Syed, S.N., et al. (2009). Eur. J. Immunol. 39(12):3343-3356).
免疫原
Recombinant mouse FcgRIV extracellular domain fused to mouse IgG1 Fc fragment (Nimmerjahn, F., et al. (2005). Immunity. 23(1):41-51).
應用
Flow Cytometry Analysis: 20 µg/mL from a representative lot, when added prior to APC-conjugated 9E9, blocked the staining of FcgRIV-positive granulocytes and monocytes by APC-conjugated 9E9 (Courtesy of Jeanette Leusen, Ph.D, University Medical Center, Utrecht, Netherlands).
Flow Cytometry Analysis: A representative lot detected FcgRIV-positive granulocytes and monocytes from wild-type, but not Fcgr4-knockout mice (Nimmerjahn, F., et al. (2010). Proc. Natl. Acad. Sci. U. S. A. 107(45):19396-19401).
Flow Cytometry Analysis: A representative lot, pre-conjugated with Alexa Fluor™ 647, immunostained murine macrophages, neutrophils, dendritic cells, but not FcgRIII-expressing NK cells. Clone 9E9 also stained a FcgRIV-negative, B220-positive peripheral blood cell population (Syed, S.N., et al. (2009). Eur. J. Immunol. 39(12):3343-3356).
Neutralizing Analysis: A representative lot, when administered (400-500 µg) via i.v. injection prior to anti-RBC 34-3C-IgG2b (50 µg) i.p. injection, prevented anemia (RBC depletion) induction by 34-3C-IgG2b. Clone 9E9 also prevented immunocomplex (IC) challenge-induced alveolar RBC & PMN infiltration in IC-sensitized mice (Syed, S.N., et al. (2009). Eur. J. Immunol. 39(12):3343-3356).
Neutralizing Analysis: A representative lot suppressed both wild-type and Fcgr1-/Fcgr3-knockout mouse peritoneal macrophages-mediated phogocytosis of red blood cells (RBCs) opsonized with anti-RBC IgG2b, but not anti-RBC IgG2a (Syed, S.N., et al. (2009). Eur. J. Immunol. 39(12):3343-3356).
Neutralizing Analysis: A representative lot prevented immune complex binding-induced activation of Fcgr3-deficient mouse neutrophils (Jakus, Z., et al. (2008). J. Immunol. 180(1):618-629).
Neutralizing Analysis: A representative lot, when administered (200 µg) via i.v. injection 30 min prior to anti-platelet 6A6-IgG2a Fc fusion (3.5 µg) injection, reduced the extend of thrombocytopenia (platelet depletion) induction by 6A6-IgG2a (Nimmerjahn, F., et al. (2005). Immunity. 23(1):41-51).
Flow Cytometry Analysis: A representative lot detected FcgRIV-positive granulocytes and monocytes from wild-type, but not Fcgr4-knockout mice (Nimmerjahn, F., et al. (2010). Proc. Natl. Acad. Sci. U. S. A. 107(45):19396-19401).
Flow Cytometry Analysis: A representative lot, pre-conjugated with Alexa Fluor™ 647, immunostained murine macrophages, neutrophils, dendritic cells, but not FcgRIII-expressing NK cells. Clone 9E9 also stained a FcgRIV-negative, B220-positive peripheral blood cell population (Syed, S.N., et al. (2009). Eur. J. Immunol. 39(12):3343-3356).
Neutralizing Analysis: A representative lot, when administered (400-500 µg) via i.v. injection prior to anti-RBC 34-3C-IgG2b (50 µg) i.p. injection, prevented anemia (RBC depletion) induction by 34-3C-IgG2b. Clone 9E9 also prevented immunocomplex (IC) challenge-induced alveolar RBC & PMN infiltration in IC-sensitized mice (Syed, S.N., et al. (2009). Eur. J. Immunol. 39(12):3343-3356).
Neutralizing Analysis: A representative lot suppressed both wild-type and Fcgr1-/Fcgr3-knockout mouse peritoneal macrophages-mediated phogocytosis of red blood cells (RBCs) opsonized with anti-RBC IgG2b, but not anti-RBC IgG2a (Syed, S.N., et al. (2009). Eur. J. Immunol. 39(12):3343-3356).
Neutralizing Analysis: A representative lot prevented immune complex binding-induced activation of Fcgr3-deficient mouse neutrophils (Jakus, Z., et al. (2008). J. Immunol. 180(1):618-629).
Neutralizing Analysis: A representative lot, when administered (200 µg) via i.v. injection 30 min prior to anti-platelet 6A6-IgG2a Fc fusion (3.5 µg) injection, reduced the extend of thrombocytopenia (platelet depletion) induction by 6A6-IgG2a (Nimmerjahn, F., et al. (2005). Immunity. 23(1):41-51).
This armenian hamster monoclonal Anti-FcgRIV (CD16-2) Antibody, clone 9E9, Cat. No. MABF853 is validated for use in Flow Cytometry and Neutralization.
品質
Evaluated by Flow Cytometry in RAW264.7 cells.
Flow Cytometry Analysis: 0.1 µg of this antibody detected FcgRIV on the surface of one million RAW264.7 murine macrophages.
Flow Cytometry Analysis: 0.1 µg of this antibody detected FcgRIV on the surface of one million RAW264.7 murine macrophages.
標靶描述
28.39/28.37/28.38 kDa (UniProt Q3TC44/Q8R2R4/Q8R477) calculated.
外觀
Format: Purified
Purified armenian hamster IgG in PBS without azide.
其他說明
Concentration: Please refer to lot specific datasheet.
法律資訊
ALEXA FLUOR is a trademark of Life Technologies
未找到合适的产品？
试试我们的产品选型工具.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门