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Merck
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主要文件

MABF471

Sigma-Aldrich

Anti-Ly-6G Antibody (mouse), PerCP-Cy5.5, clone 1A8

clone 1A8, 0.2 mg/mL, from rat

别名:

Lymphocyte antigen 6G, Ly-6G, Ly-6G.1

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rat

质量水平

偶联物

PerCP-Cy5.5

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

1A8, monoclonal

种属反应性

mouse

浓度

0.2 mg/mL

技术

flow cytometry: suitable

同位素/亚型

IgG2aκ

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

mouse ... Ly6G(546644)

一般描述

The 1A8 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the 1A8 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: for identification of Ly-C, an alternative antibody, clone RB6-8C5, has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C.
This rat monoclonal antibody Anti-Ly-6G (mouse), PerCP-Cy5.5 & clone 1A8 validated for use in Flow Cytometry for the detection of Ly-6G.

质量

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected Ly-6G in one million C57Bl/6 bone marrow cells.

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储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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