MABF3113

Anti-NKp30/CD337 Antibody, clone F252

• 别名: Activating natural killer receptor p30, CD337, NK-p30, Natural cytotoxicity triggering receptor 3, Natural killer cell p30-related protein
• UNSPSC代码: 12352203
• NACRES: NA.43

属性

• 生物来源: mouse
• 质量水平: 200
• 抗体形式: purified antibody
• 抗体产品类型: primary antibodies
• 克隆: F252, monoclonal
• 分子量: calculated mol wt 21.59 kDa; observed mol wt ~N/A kDa
• 纯化方式: affinity chromatography
• 种属反应性: human
• 包装: antibody small pack of 100
• 技术: flow cytometry: suitable; inhibition assay: suitable; neutralization: suitable
• 同位素/亚型: IgMκ
• 表位序列: Extracellular domain
• Protein ID登记号: NP_001138938
• UniProt登记号: O14931
• 储存温度: -10 to -25°C
• 基因信息: human ... NCR3(259197)

说明

特异性

Clone F252 is a mouse monoclonal antibody that detects Natural cytotoxicity triggering receptor 3 (NKp30/CD337). It targets an epitope within the extracellular domain.

免疫原

Activated human natural killer (NK) cells.

应用

Quality Control Testing

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis: 1 µg of this antibody detected NKp30 in one million Human peripheral blood mononuclear cells (PBMC).

Tested Applications

Flow Cytometry Analysis: A representative lot detected NKp30 (CD337) in Flow Cytometry application. (Meazza, R., et al. (2020). Cancers (Basel). 12(8):2187; Salome, B., et al. (2022). Cancer Cell. 40(9):1027-1043.e9).

Inhibition: A representative lot Inhibited the lysis of antigen presenting cells (APC) and blocked the expression of CD83, CD86.(Spaggiari, G.M., et al. (2001). Eur J Immunol.;31(6):1656-65; Vitale, M., et al. (2005). Blood. 106(2):566-71).

Flow Cytometry Analysis: A representative lot detected NKp30 (CD337) in Human peripheral blood mononuclear cells (PBMC). (Courtesy of Dr. Raffaella Meazza, IRCCS Ospedale Policlinico San Martino, Genova, Italy).

Neutralizing: A representative lot neutralized the receptor function of NKp30 (CD337). (Witkowski, M., et al. (2021). Nature. 600(7888):295-301).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

目标描述

Natural cytotoxicity triggering receptor 3 (UniProt: O14931; also known as Activating natural killer receptor p30, Natural killer cell p30-related protein, NK-p30, NKp30, CD337) is encoded by the NCR3 (also known as 1C7, LY117) gene (Gene ID: 259197) in human. NKp30 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 19-135), a transmembrane domain (aa 136-156), and a cytoplasmic domain (aa 157-201). It is selectively expressed by all resting and activated NK cells. Its weaker expression is observed in spleen. It serves as a receptor of NK cells. It is activated by binding of extracellular ligands including BAG6 and NCR3LG1 and stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It cooperates with NKp46 and/or NKp44 in inducing NK-mediated cytotoxicity against the majority of target cells. It is also reported to control NK cells cytotoxicity against tumor cells. Its interaction with BAG6 promotes myeloid dendritic cells (DC) maturation, both through killing DCs that fail to achieve a mature phenotype and by inducing the release of TNF- and interferon- by NK cells that promote DC maturation. Masking of NKp30 with specific monoclonal antibodies is reported to reduce maturation of DCs cocultured with NK cells. Addition of supernatant from NK cells stimulated via NKp30 is shown to induce DC maturation. Six isoforms of NKp30 have been described that are produced by alternative splicing. (Ref.: Vitale, M., et al. (2005). Blood. 106(2); 566-571; Pende, D., et al. (1999). J. Exp. Med. 190(10); 1505-1516).

外形

Purified mouse monoclonal antibody IgM in PBS without preservatives.

重悬

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 2
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable