MABE982

Anti-RBP-Jk, clone 1F1 Antibody

clone 1F1, from rat

• 别名: Recombining binding protein suppressor of hairless, CBF-1, J kappa-recombination signal-binding protein, RBP-J kappa, RBP-J, RBP-JK, Renal carcinoma antigen NY-REN-30
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rat
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 1F1, monoclonal
• 物種活性: Drosophila, human
• 技術: ChIP: suitable; electrophoretic mobility shift assay: suitable; immunoprecipitation (IP): suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_005340
• UniProt登錄號: Q06330
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... RBPJ(3516)

说明

一般說明

RBP-jk, Recombinant signal binding protein for immunoglobin kappa J region, is a human homolog for the Drosophila gene, suppressor of hairless. RBP-jk is located in the nucleus and is a 60 kDa monomeric protein that is used as a promoter region in notch signaling. Notch signaling is important in cell to cell communication which allows "a differentiating cell to suppress the same differentiation fate in a neighboring cell (the receiving cell). This ensures that the correct number of cells are distributed to distinct cell fates." When RBP-jk is not associated with Notch proteins, it acts as a transcriptional repressor. A disease associated with RBP-jk is the Epstein Barr virus.

免疫原

Recombinant protein corresponding to human RBP-Jk.

應用

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using Lymphoblastoid cell line.

Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using DG75 cells.

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP-Jk using Hippocampal NSC′s (Ehm, O., et al. (2010). Journal of Neuroscience. 30(41):13794-18707).

Electrophoretic Mobility Shift Assay: A representative lot detected RBP-Jk using Hippocampal lysate (Ehm, O., et al. (2010). Journal of Neuroscience. 30(41):13794-18707).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-RBP-Jk, clone 1F1 Antibody is validated for use in Immunoprecipitation and Chromatin Immunoprecipitation (ChIP) and Electrophoretic Mobility Shift Assay for the detection of RBP-Jk.

品質

Evaluated by Immunoprecipitation in HeLa cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated RBP-Jk in 0.5 mg of HeLa cell lysate.

標靶描述

~55 kDa observed

外觀

Protein G Purified

Format: Purified

Purified rat IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable