MABE979

Anti-Histone Demethylase JARID1C Antibody, clone 2B4.2

clone 2B4.2, from mouse

• 别名: Lysine-specific demethylase 5C, Histone demethylase JARID1C, Jumonji/ARID domain-containing protein 1C, Protein SmcX, Protein Xe169
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 2B4.2, monoclonal
• 物種活性: human
• 物種活性（以同源性預測）: mouse (based on 100% sequence homology)
• 技術: western blot: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_001140174
• UniProt登錄號: P41229
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... KDM5C(8242) ; mouse ... Kdm5C(20591)

说明

一般說明

Lysine-specific demethylase 5C (UniProt P41229; also known as Histone demethylase JARID1C, Jumonji/ARID domain-containing protein 1C, Protein SmcX, Protein Xe169) is encoded by the KDM5C (also known as DXS1272E, JARID1C, MRXSCJ, SMCX, XE169) gene (Gene ID 8242) in human. RBP2 (JARID1A), PLU-1 (JARID1B), KDM5C (JARID1C), and SMCY (JARID1D) constitute the Jumonji/ARID (JARID) subfamily of JmjC-containing proteins. KDM5C/JARID1C catalyzes the demethylation of di- and trimethylated lysine 4 of histone 3 in a Fe(II) and α-ketoglutarate-dependent manner. JARID1 catalytic activity is medidated by its JmjC domain (a.a. 468-634), while the JmjN domain (a.a. 14-55) and the C5HC2 zinc finger (a.a. 326-372 & 1187-1248) are both important in assisting JARID1C catalytic activity. JARID1C also contains an AT-rich domain-interacting domain (ARID) that binds DNA and its N-terminal PHD (plant homeodomain) finger (a.a. 326-372) is reported to bind trimethylated lysine 9 on histone 3 (H3K9me3). JARID1C interacts with PCNA through its PCNA-interaction protein (PIP) motif and JARID1C is among 485 chromatin factors enriched at nascent versus mature post-replicative chromatin, indicating a potential transcription-independent role during DNA replication.

特異性

Clone 2B4.2 targets an N-terminal region sequence present in all human and murine JARID1C/KDM5C spliced isoforms reported by UniProt (P41229 and P41230).

免疫原

Epitope: N-terminal region.

KLH-conjugated linear peptide corresponding to an N-terminal region sequence of human JARID1C.

應用

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histone Modifying Proteins

This Anti-Histone Demethylase JARID1C Antibody, clone 2B4.2 is validated for use in Western Blotting for the detection of Histone Demethylase JARID1C.

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected histone demethylase JARID1C in 10 µg of HeLa cell lysate.

標靶描述

~180 kDa observed. 175.7/175.3 kDa (human/mouse isoform 1), 175.4/175.0 kDa (human/mouse isoform 2), 170.6/170.2 kDa (human/mouse isoform 3), 155.1/175.6 kDa (human isoform 4/5) calculated. Uncharacterized band(s) may appear in some lysates.

外觀

Protein G purified.

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1