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生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
162C, monoclonal
分子量
calculated mol wt 40.29 kDa
observed mol wt ~40 kDa
純化經由
using protein G
物種活性
human
包裝
antibody small pack of 100 μg
技術
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG1κ
表位序列
Unknown
Protein ID登錄號
UniProt登錄號
運輸包裝
ambient
目標翻譯後修改
unmodified
基因資訊
vaccinia virus ... N(43740575)
一般說明
Hypoxia-inducible factor 1-alpha inhibitor (UniProt: Q9NWT6; also known as EC:1.14.11.30, Factor inhibiting HIF-1, FIH-1, Hypoxia-inducible factor asparagine hydroxylase) is encoded by the HIF1AN (also known as FIH1) gene (Gene ID: 55662) in human. FIH is a widely expressed, Fe(II)-dependent, homodimeric protein that in the presence of oxygen hydroxylates HIF1 at Asn 803 in the C-terminal transactivation domain. This hydroxylation prevents interaction of HIF1 activation domain with the CH-1 domain of the transcriptional co-activator p300 and reduces the transcriptional activity of HIF1 . Its homodimerization is reported to be essential for its catalytic activity. FIH is mainly cytosolic, but its interaction with NOTCH1 results in its nuclear localization and its interaction with ABPA3 results in its perinuclear localization in macrophages. Silencing of FIH gene by RNA Interference is shown to up-regulate HIF-dependent target gene expression in normoxia. (Ref.: Soilleux, EJ., et al. (2005). Histopathology. 47(6); 602-610; Stolze, IP., et al. (2004). J. Biol. Chem. 279(41); 42719-42725; Hewitson, KS., et al. (2002). J. Biol. Chem. 277(29); 26351-26355)
特異性
Clone 162C is a mouse monoclonal antibody that specifically detects Hypoxia-inducible factor 1-alpha inhibitor (FIH).
免疫原
His-tagged full-length recombinant human Hypoxia-inducible factor 1-alpha inhibitor (FIH) expressed in E. coli.
應用
Quality Control Testing
Evaluated by Western Blotting in MCF-7 cell lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected FIH in MCF-7 cell lysates.
Tested Applications
Western Blotting Analysis: A representative lot detected FIH in Western Blotting applications (Stolze, I.P., et al. (2004). J Biol Chem. 279(41): 42719-25).
Immunohistochemistry Applications: A representative lot detected FIH in Immunohistochemistry applications (Soilleux, E.J., et al. (2005). Histopathology. 47(6): 602-10).
Immunofluorescence Analysis: A representative lot detected FIH in Immunofluorescence applications (Stolze, I.P., et al. (2004). J Biol Chem. 279(41): 42719-25).
Immunocytochemistry Analysis: A representative lot detected FIH in Immunocytochemistry applications (Stolze, I.P., et al. (2004). J Biol Chem. 279(41): 42719-25).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in MCF-7 cell lysates.
Western Blotting Analysis: A 1:500 dilution of this antibody detected FIH in MCF-7 cell lysates.
Tested Applications
Western Blotting Analysis: A representative lot detected FIH in Western Blotting applications (Stolze, I.P., et al. (2004). J Biol Chem. 279(41): 42719-25).
Immunohistochemistry Applications: A representative lot detected FIH in Immunohistochemistry applications (Soilleux, E.J., et al. (2005). Histopathology. 47(6): 602-10).
Immunofluorescence Analysis: A representative lot detected FIH in Immunofluorescence applications (Stolze, I.P., et al. (2004). J Biol Chem. 279(41): 42719-25).
Immunocytochemistry Analysis: A representative lot detected FIH in Immunocytochemistry applications (Stolze, I.P., et al. (2004). J Biol Chem. 279(41): 42719-25).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-FIH, clone 162C, Cat. No. MABE2031, is a mouse monoclonal antibody that detects Factor inhibiting HIF-1 (FIH) and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
外觀
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Recommended storage: +2°C to +8°C.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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